Escherichia coli Library
The Center holds the following collections, which were transferred from the Nara Institute of Science and Technology (NAIST, Developer: Dr. Hirotada Mori). The collections can be used for comprehensive research on Escherichia coli, a model prokaryote organism.
① Keio Collection※1
a set of single-gene deletion mutants
② ASKA Clone 骋贵笔(+)※1
a set of ORFs cloned into the high copy number plasmid with GFP
③ ASKA Clone 骋贵笔(-)※1
a set of ORFs cloned into the high copy number plasmid without GFP
④ Gateway Entry Clone library※1
a set of clone library for transfer of the ORFs to other vectors
⑤ TransBac library
a set of ORFs cloned into the low copy number plasmid
⑥ ASKA barcode deletion Collection
a set of single-gene deletion mutants with barcode sequence
⑦ ASKA barcode deletion Collection with Sm resistance
a set of single-gene deletion mutants with barcode sequence and streptomycin resistance
※1 : For the use of ① – ④ in academic research at non-profit institutions, please contact .